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肿瘤坏死因子受体相关因子1抗体上海雅吉生物科技有限公司

性能参数

产品名称: 肿瘤坏死因子受体相关因子1抗体
英文名称: TRAF1
抗体货号: 1212
产品规格: 50ul/100ul/200ul
级    别: 生化级,
产品产地: 上海
品牌商标: 雅吉
价    格: 询价
抗原: KLH conjugated synthetic peptide derived from human TGF beta R2:241-330/567
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Rabbit, )
Alexa Fluor 488 标记 Alexa Fluconjugate:Alexa Fluor 488 标记 Alexa Flu
应用范围: ELISA IHC-P IHC-F IF
更新时间: 2020/11/19 13:18:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

  

中文名称 肿瘤坏死因子受体相关因子1抗体
别    名 4732496E14Rik; EBV induced protein 6; Epstein-Barr virus-induced protein 6; Tnf receptor associated factor 1; TRAF 1; TRAF1_HUMAN.  

 

研究领域 肿瘤  免疫学  信号转导  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, Rabbit, )
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 46kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TRAF1:301-416/416 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. [provided by RefSeq].

Function:
Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.

Subunit:
Homotrimer. Heterotrimer with TRAF2. Interacts with TNFRSF1A/TNFR1, TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13C, TNFRSF18/AITR, TNFRSF17/BCMA, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1, TANK/ITRAF, TRAIP and RIPK2. Interacts with BIRC2 and BIRC3 N-terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2. Interacts with NFATC2IP, TRAFD1 and with HIVEP3 (By similarity). Interacts with MAP3K14.

Post-translational modifications:
Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation.

Similarity:
Contains 1 MATH domain.

SWISS:
Q13077

Gene ID:
7185

Database links:

Entrez Gene: 700583 Cow

Entrez Gene: 7185 Human

Entrez Gene: 22029 Mouse

Omim: 601711 Human

SwissProt: Q13077 Human

SwissProt: P39428 Mouse

Unigene: 531251 Human

Unigene: 239514 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

信号传导(Signaling Intermediates)
TRAF1是TRAFs分子中最有可能出现差异表达的分子之一;TRAF1分子不包含其它TRAFs分子皆有的环结构-没有重排的锌指结构。TNFR1在介导细胞凋亡信号及在肿瘤细胞的凋亡中发挥着一定的作用。
产品图片 Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF1) Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF1) Polyclonal Antibody, Unconjugated (bs-1212R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAF1 Polyclonal Antibody, Unconjugated(bs-1212R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse tumor tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAF1 Polyclonal Antibody, Unconjugated(bs-1212R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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