高尔基体膜蛋白GP73抗体
- 来源:上海雅吉生物科技有限公司
- 时间: 2020/11/9
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| 中文名称 | 高尔基体膜蛋白GP73抗体 |
| 别 名 | Chromosome 9 open reading frame 155; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2; Golgi protein 73 kD; golgi protein 73kD; GOLM 1; GOLM1; GOLPH 2; GOLPH2; GP 73; GP73; PSEC0257; bA379P1.3; C9orf155. |
| 研究领域 | 肿瘤 信号转导 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 45kDa |
| 细胞定位 | 细胞浆 细胞膜 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GOLPH2:21-120/401 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | GOLPH2 is widely expressed. Expression levels are high in the colon, prostate, trachea and stomach; expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. GOLPH2 is predominantly expressed by cells of the epithelial lineage. It is expressed at a low level in normal liver, though expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non viral causes (alcohol induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte derived syncitial giant cells. GOLPH2 is constitutively expressed by biliary epithelial cells but not by hepatocytes. Function: Unknown. Cellular response protein to viral infection. Subcellular Location: Golgi apparatus, cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. Tissue Specificity: Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. Post-translational modifications: Glycosylated. Phosphorylation sites are present in the extracelllular medium. Similarity: Belongs to the GOLM1/CASC4 family. SWISS: Q8NBJ4 Gene ID: 51280 Database links: Entrez Gene: 51280 Human Entrez Gene: 105348 Mouse Entrez Gene: 306725 Rat Omim: 606804 Human SwissProt: Q8NBJ4 Human SwissProt: Q91XA2 Mouse Unigene: 494337 Human Unigene: 171335 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:A549(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- TBX1 (bs-4887R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD  (human/cell line)A431 lysates probed with GOLPH2 Polyclonal Antibody, Unconjugated (Catalog #bs-4887R) at 1:300 overnight at 4ⅹC. Followed by a conjugated secondary antibody (Secondary Catalog #926-32211) at 1:10000 for 60 min at 37ⅹC. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (GOLPH2) Polyclonal/MonoclonalAntibody, Unconjugated (bs-4887R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (GOLPH2) Polyclonal/MonoclonalAntibody, Unconjugated (bs-4887R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. |
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