磷酸化微管相关蛋白抗体

  • 来源:上海雅吉生物科技有限公司
  • 时间: 2020/11/9
  • 浏览人数: 645

      

    中文名称 磷酸化微管相关蛋白抗体
    别    名 Tau(Phospho-Thr522); MAPT(phospho T522); p-Tau protein (phospho-Thr522); phospho-Tau (Thr205); Tau(Phospho-Thr205); MAPT; Microtuble-associted protein Tau; AI413597; AW045860; DDPAC; Disinhibition dementia parkinsonism amyotrophy complex; FLJ31424; FTDP 17; FTDP17; G Protein beta 1 gamma 2 subunit interacting factor 1; G protein beta1/gamma2 subunit interacting factor 1; MAPTL; MGC134287; MGC138549; MGC156663; Microtubule associated protein tau isoform 4; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; PHF tau; PHF-tau; PPND; pTau; RNPTAU; Tauopathy and respiratory failure, included; TAU_HUMAN.  
    产品类型 磷酸化抗体 
    研究领域 细胞生物  免疫学  神经生物学  信号转导  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat,  (predicted: Dog, Horse, Rabbit, )
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 52/79kDa
    细胞定位 细胞浆 细胞膜 
    性    状 Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Tau protein around the phosphorylation site of Thr522:PG(p-T)PG 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    PubMed PubMed
    产品介绍 Tau proteins are important Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tau proteins subcellular located in the axons of neurons, in the cytoso l and in association with plasma membrane components. It expressed in neurons. PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

    Function:
    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

    Subunit:
    Interacts with PSMC2 through SQSTM1. Interacts with SQSTM1 when polyubiquitinated. Interacts with FKBP4. Binds to CSNK1D. Interacts with SGK1.

    Subcellular Location:
    Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Note=Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.

    Tissue Specificity:
    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

    Post-translational modifications:
    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1: CDK1, CDK5, GSK3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in the form associated with paired helical filaments (PHF-tau)), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Phosphorylation decreases with age. Phosphorylation within tau/MAP's repeat domain or in flanking regions seems to reduce tAU/MAP's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis. Phosphorylation at Ser-548 by GSK3B reduces ability to bind and stabilize microtubules. Phosphorylation at Ser-579 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylated at Ser-554, Ser-579, Ser-602, Ser-606 and Ser-669 by PHK. Phosphorylation at Ser-214 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. There is a reciprocal down-regulation of phosphorylation and O-GlcNAcylation. Phosphorylation on Ser-717 completely abolishes the O-GlcNAcylation on this site, while phosphorylation on Ser-713 and Ser-721 reduces glycosylation by a factor of 2 and 4 respectively. Phosphorylation on Ser-721 is reduced by about 41.5% by GlcNAcylation on Ser-717.

    DISEASE:
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.

    Similarity:
    Contains 4 Tau/MAP repeats.

    SWISS:
    P10636

    Gene ID:
    4137

    Database links:

    Entrez Gene: 281296 Cow

    Entrez Gene: 4137 Human

    Entrez Gene: 17762 Mouse

    Entrez Gene: 29477 Rat

    Omim: 157140 Human

    SwissProt: P29172 Cow

    SwissProt: P10636 Human

    SwissProt: P10637 Mouse

    SwissProt: P19332 Rat

    Unigene: 101174 Human

    Unigene: 1287 Mouse

    Unigene: 2455 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    P-tau蛋白是脑内神经元细胞支架蛋白之一。其正常功能是促进微管蛋白组成微管,并维持已形成微管的稳定性。参与维持细胞形态、信息传递、细胞分裂等重要生物学过程,是轴突生长发育和神经元极性形成的不可缺少因素。近年来发现tau蛋白与一些中枢神经系统变性疾病密切相关,尤其是神经Tau具有启动微管系统的装配以及稳定微管系统的作用,该蛋白的错误折叠与老年性痴呆等神经退行性疾病密切相关。
    产品图片 Sample:
    BV-2(Mouse) Cell Lysate at 30 ug
    Primary: Anti-phospho-Tau (Thr522) (bs-10113R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52/79 kD
    Observed band size: 52/129 kD
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Cerebrum (Rat) Lysate at 40 ug
    Primary: Anti-phospho-Tau (Thr522) (bs-10113R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 52/79 kD
    Observed band size: 52 kD
    Sample:
    Cerebrum (Mouse) Lysate at 40 ug
    Cerebrum (Rat) Lysate at 40 ug
    Cerebellum (Mouse) Lysate at 40 ug
    Cerebellum (Rat) Lysate at 40 ug
    Primary: Anti-phospho-Tau (Thr522) (bs-10113R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 50-70 kD
    Observed band size: 50 kD
    Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tau (Thr522)) Polyclonal Antibody, Unconjugated (bs-10113R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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