磷酸化谷氨酸受体1抗体

  • 来源:上海雅吉生物科技有限公司
  • 时间: 2020/11/9
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    中文名称 磷酸化谷氨酸受体1抗体
    别    名 p-GluR-1(Ser 863); p-GluR1(Ser863); Glutamate Receptor 1 (phospho S863); Glutamate Receptor 1 (phospho Ser863); GLUR 1; GLUR A; AMPA 1; GluR-1; AMPA selective glutamate receptor 1; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Glutamate receptor ionotropic; Glutamate receptor, ionotropic, AMPA 1; Gria 1; Gria1; GRIA1_HUMAN; HBGR1; MGC133252; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243.  
    产品类型 磷酸化抗体 
    研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  转录调节因子  细胞膜受体  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 98kDa
    细胞定位 细胞浆 细胞膜 
    性    状 Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthesised phosphopeptide derived from human GluR1 around the phosphorylation site of Ser863:RN(p-S)GA 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    PubMed PubMed
    产品介绍 Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

    Function:
    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

    Subunit:
    Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with SYNDIG1 and GRIA2. Interacts with LRFN. Interacts with HIP1 and RASGRF2. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5. Interacts with CNIH2 and CACNG2.

    Subcellular Location:
    Cell membrane. Endoplasmic reticulum membrane.

    Tissue Specificity:
    Widely expressed in brain.

    Post-translational modifications:
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

    Similarity:
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.

    SWISS:
    P42261

    Gene ID:
    2890

    Database links:

    Entrez Gene: 2890 Human

    Entrez Gene: 14799 Mouse

    Entrez Gene: 50592 Rat

    Omim: 138248 Human

    SwissProt: P42261 Human

    SwissProt: P23818 Mouse

    SwissProt: P19490 Rat

    Unigene: 519693 Human

    Unigene: 4920 Mouse

    Unigene: 29971 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
     
    产品图片 Sample:
    Cerebellum (Mouse) Lysate at 40 ug
    Primary: Anti-phospho-GluR1 (Ser863) (bs-11632R) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 98 kD
    Observed band size: 128 kD
    Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GluR1 (Ser863)) Polyclonal Antibody, Unconjugated (bs-11632R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human glioma tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GluR1 (Ser863)) Polyclonal Antibody, Unconjugated (bs-11632R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GluR1 (Ser863)) Polyclonal Antibody, Unconjugated (bs-11632R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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