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ABI荧光定量A25742北京智杰方远科技有限公司

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产品名称: ABI荧光定量A25742
英文名称: PowerUp™ SYBR™ Green Master Mix
产品货号: A25742
产品规格: 5 mL
试剂级别: 分子生物学级
产品产地: USA
产品商标: Applied Biosystems
价    格: 询价元
保存与运输: 4度
现货状态: 现货
产品资料: 实验方法技术资料
更新时间: 2022/5/17 9:08:00
浏览人数:377
诚信指数:1017点
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使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

北京智杰方远科技有限公司
地址:
北京市海淀区马连洼北路九号东居兴业写字楼
邮编:
100196
电话:
010-82897201 15810200961
传真:
010-62894435
联系人:
张女士
所在区域:
北京·北京
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公司展台:
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产品详细描述

ABI荧光定量PCR  A25742北京智杰方远科技现货特价促销!

 

 

品 名

品牌

货号

规格

促销价

PowerUp™ SYBR™ Green Master Mix

ABI

A25742

 

5 mL

900

PowerUp™ SYBR™ Green Master Mix

ABI

A25777

5 x 5 mL

询价

PowerUp™ SYBR™ Green Master Mix

ABI

A257778

 

10 x 5 mL

询价

规格

Detection Method:

SYBR

For Use With (Equipment):

7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System

Reaction Speed:

Fast or Standard

PCR Method:

qPCR

Sample Type (General):

DNA

Number of Reactions:

500 reactions

Polymerase:

Dual-Lock™ Taq DNA Polymerase

Product Line:

PowerUP™, SYBR®

Product Size:

5 mL

Shipping Condition:

Wet Ice

内容及储存

The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500 20-μL reactions (10 μL Master Mix per reaction).

Store at 2–8°C.

描述

PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.

Broad instrument compatibility
PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.

Learn more about PowerUp™ SYBR® Green Master Mix >

 

 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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